rabbit anti-gsk3β  (Cell Signaling Technology Inc)

Journal: Nutrients

Article Title: Artemisiae Iwayomogii Herba Protects Dopaminergic Neurons Against 1-Methyl-4-phenylpyridinium/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Neurotoxicity in Models of Parkinson's Disease.

doi: 10.3390/nu17101672

Figure Lengend Snippet: Figure 3. AIK treatment stimulates the Akt/GSK3β/Nrf2 signaling pathway to induce the expression of HO-1 in PC12 cells. Cells were treated with AIK at concentrations of 30 and 100 µg/mL and then incubated with 1 mM of MPP+ for 4 h. (A) Representative band images and the quantifications of (B) p-Akt normalized to t-Akt and (C) GSK3β normalized to t-GSK3β. (D) Representative band images and quantifications of (E) cytoplasmic Nrf2, (F) nuclear Nrf2, and (G) HO-1. Cytoplasmic Nrf2 and HO-1 were normalized to β-actin, and nuclear Nrf2 was normalized to PCNA. Values are indicated as the mean ± S.E.M. Data were analyzed by one-way ANOVA, followed by Dunnett’s multiple-comparison test. ## p < 0.01 compared to the control group; ** p < 0.01 and * p < 0.05 compared to the MPP+-treated group.

Article Snippet: Rabbit anti-protein kinase B (Akt, #9272), anti-phospho-Akt (Ser473, #4060), anti-glycogen synthase kinase-3β (GSK3β, #12456), and phospho-GSK3β (Ser9, #5558) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Incubation, Comparison, Control